Born on June 5, 1982, in Seongnam, Gyeonggi-do, South Korea, Yoo In-na initially pursued a career in music, joining an entertainment agency as a trainee singer in 1998. After several years, she transitioned to acting, making her debut with a supporting role in the television series High Kick! Through the Roof (2009–2010). She gained further recognition with her role in Secret Garden (2010). Yoo In-na's breakthrough came as the lead in Queen In-hyun's Man (2012), followed by notable performances in My Love from the Star (2013–2014), Guardian: The Lonely and Great God (2016–2017), and Touch Your Heart (2019). Apart from acting, she served as the DJ for the popular radio program Let's Crank Up the Volume from 2011 to 2016. In 2023, Yoo In-na donated 30 million won to aid victims of the Turkey-Syria earthquake, demonstrating her commitment to philanthropy.